ForenSeq mtDNA Control Region Kit

The ForenSeq mtDNA Control Region Kit offers convenient, efficient library prep for interrogating the control region of the mitochondrial genome (mtGenome) with minimal DNA input and optimum sensitivity. Leveraging proven ForenSeq chemistry, the kit produces a concise data set from the hypervariable I and II (HVI and HVII) regions for direct upload to CODIS. Multiplexing capability boosts operational efficiency without increases to costs or time.

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Description

Target size  1200 bp mtDNA control region
Sample type gDNA and mtDNA extracted from hair, bone, teeth, and buccal swabs
DNA input recommendation 100 pg gDNA per sample
Multiplexing capacity 3–48 samples per run
Number of primers > 120
Number of amplicons 18
Average amplicon size 118 bp
Amplicon overlap ≥ 3 bp
Total library prep time 7 hours and 45 minutes
Hands-on time 90 minutes

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