ForenSeq DNA Signature Prep Kit

Generate more comprehensive DNA profiles for up to 96 DNA samples with fewer than two hours of hands-on time using our simple, plate-based workflow. The ForenSeq DNA Signature Prep Kit is the only next-generation sequencing (NGS)-based short-tandem repeat (STR) sequencing chemistry approved for upload to the National DNA Index System (NDIS) for casework. Combining over 200 markers, including autosomal, X- and Y-STRs, identity-informative and phenotypic-informative SNPs into a single, streamlined workflow, the kit eliminates the need to run multiple STR tests.

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Description

Sample type gDNA, buccal swabs, FTA card
Recommended input for gDNA 1 ng per sample
Recommended input for FTA card punch 1.2 mm per sample
Multiplexing capacity 8–96 libraries per run
Locus multiplexing capability Simultaneous analysis of ~200 genetic markers
Number of primer mixes 2 (DPMA and DPMB)
Primer mix content DPMA: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs

DPMB: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs, 22 phenotypic SNPs, 56 biogeographical ancestry SNPs

Short amplicon detection High concentration of markers < 200 bp
Low-level mixture detection Detects minor contributors at < 5%
Total library prep time 7 hours and 15 minutes
Hands-on time 1 hour and 45 minutes
Total sequencing time ~30 hours with the MiSeq FGx Reagent Kit

~22 hours with the MiSeq FGx Reagent Micro Kit

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